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001			233455
003			ES-VaUE
005			20221220020500.0
007			cr nn 008mamaa
008			191126s2020 xxu| s |||| 0|eng d
020			_a9781071601679
024	7		_a10.1007/978-1-0716-0167-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aInositol Phosphates _bMethods and Protocols _cedited by Gregory J. Miller.
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (X, 185 páginas) _b35 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2091
505	0		_aA Short Historical Perspective of Methods in Inositol Phosphate Research -- Quantitation of Inositol Phosphates by HPLC-ESI-MS -- Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation -- Detection of Inositol Phosphates by Split PH Domains -- Dictyostelium discoideum as a Model to Study Inositol Pyrophosphate and Inorganic Polyphosphate -- Characterising Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family -- Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells -- Back-pyrophosphorylation Assay to Detect in vivo InsP7-dependent Protein Pyrophosphorylation in Mammalian Cells -- Analyses of Ligand Binding to IP3 receptors using Fluorescence Polarization -- Investigating the InsP3 Receptor in Living Cells by Caged InsP3 -- Optical Measurement of Synaptic Vesicle Fusion and its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons -- Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate -- Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle-vesicle Fusion Assay -- Engineering Protease Resistant and Highly Active Phytases -- Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-cleaving Tag -- A Short Historical Perspective of Methods in Inositol Phosphate Research. .
520			_aThis volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.
700	1		_aMiller, Gregory J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071601662
776	0	8	_iPrinted edition: _z9781071601686
776	0	8	_iPrinted edition: _z9781071601693
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0167-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2020
999			_c233455 _d233455