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001			233460
003			ES-VaUE
005			20221220020501.0
007			cr nn 008mamaa
008			200925s2021 xxu| s |||| 0|eng d
020			_a9781071609828
024	7		_a10.1007/978-1-0716-0982-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aListeria Monocytogenes _bMethods and Protocols _cedited by Edward M. Fox, Hélène Bierne, Beatrix Stessl.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 260 páginas) _b54 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2220
505	0		_aTraditional Methods of Analysis for Listeria monocytogenes -- MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes -- Sample Preparation for qPCR Detection of Listeria from Food -- qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay -- Serotype Assignment by Sero-Agglutination, ELISA, and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format -- Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities -- Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method -- Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Microscopy of Intracellular Listeria monocytogenes in Epithelial Cells -- Control of Listeria monocytogenes Biofilms in a Simulated Food Processing Environment -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP) -- Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources.
520			_aThis updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.
700	1		_aFox, Edward M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBierne, Hélène _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aStessl, Beatrix _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609811
776	0	8	_iPrinted edition: _z9781071609835
776	0	8	_iPrinted edition: _z9781071609842
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0982-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233460 _d233460