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020 _a9781071611821
024 7 _a10.1007/978-1-0716-1182-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHost-Fungal Interactions
_bMethods and Protocols
_cedited by Elaine Bignell.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 242 páginas)
_b39 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2260
505 0 _aDiminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans -- Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification -- CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia -- Analysis of Epithelial Cell Responses to Microbial Pathogens -- Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry -- Fungal Bioreporters to Monitor Outcomes of Blastomyces - Host Cell Interactions -- Fungal Bioreporters to Monitor Outcomes of Aspergillus - Host Cell Interactions -- Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays -- Experimental Evolution of Candida by Serial Passaging in Host Cells -- Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells -- Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras -- Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole -- Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections -- A Murine Model for Chronic A. fumigatus Airway Infections -- Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge. .
520 _aThis volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700 1 _aBignell, Elaine
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071611814
776 0 8 _iPrinted edition:
_z9781071611838
776 0 8 _iPrinted edition:
_z9781071611845
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1182-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233467
_d233467