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008			210121s2021 xxu| s |||| 0|eng d
020			_a9781071612378
024	7		_a10.1007/978-1-0716-1237-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRegulatory B Cells _bMethods and Protocols _cedited by Francesca Mion, Silvia Tonon.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIV, 471 páginas) _b90 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2270
505	0		_a Purification and characterization of murine MZ and T2-MZP cells -- Purification and immune-phenotyping of B-1 cells from body cavities of mice -- Purification and immunophenotypic characterization of murine plasma cells -- Purification of murine and human IL-10 producing B cells from different anatomical compartments -- Purification and immunophenotypic characterization of human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B cells -- Detection of IL-10 in murine B cells: in vitro and in vivo techniques -- Detection and quantification of transforming growth factor-β1 produced by murine B cells: pros and cons of different techniques -- IL-35 detection in B cells at the mRNA and protein level -- Characterization and activation of Fas Ligand producing mouse B cells and their killer exosomes -- Characterization and activity of TIM-1 and IL-10-reporter expressing regulatory B cells -- New method for the expansion of highly purified human regulatory granzyme B-expressing B cells -- Characterization of the cell surface phenotype and regulatory activity of B cell IgD low (BDL) regulatory B cells -- Use of Toll-like receptor (TLR) ligation to characterize human regulatory B cells subsets -- Use of co-cultures for the study of cellular interactions influencing B cell regulatory functions -- Use of inhibitory compounds to dissect the molecular pathways involved in regulatory B cell differentiation -- Methods to study the transcriptome of regulatory B cells -- Purification of murine IL-10+ B cells for analyses of biological functions and transcriptomics -- il-10 gene locus DNA methylation in regulatory B cells -- B cell commitment to IL-10 production: the Vert-X Il10egfp mouse -- Regulatory B cells in experimental mouse models of arthritis -- Regulatory and IgE+ B cells in allergic asthma -- Regulatory B cells in type 1 diabetes -- Analysis of regulatory B cells in experimental autoimmune uveitis -- Purification and immunophenotypic characterization of human CD24hiCD38hi and CD24hiCD27+ regulatory B cells in transplantation.
520			_a This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols aims to be useful to the scientific community and serve to clarify some unsolved aspects of Regulatory B Cells research.
700	1		_aMion, Francesca _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTonon, Silvia _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612361
776	0	8	_iPrinted edition: _z9781071612385
776	0	8	_iPrinted edition: _z9781071612392
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1237-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233471 _d233471