000			03461nam a22003615i 4500
001			233476
003			ES-VaUE
005			20221220020502.0
007			cr nn 008mamaa
008			210212s2021 xxu| s |||| 0|eng d
020			_a9781071610565
024	7		_a10.1007/978-1-0716-1056-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPericytes _bMethods and Protocols _cedited by Bruno M. Péault.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 192 páginas) _b29 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2235
505	0		_aVisualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture -- Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice -- Assessment of Pericyte Phenotype by Flow Cytometry -- Characterization of Pericyte Phenotype In Vivo by Immunohistochemistry -- A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures -- Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line Origins -- Imaging Pericytes and the Regulation of Cerebral Blood Flow -- Derivation of Pericytes from Human Pluripotent Stem Cells -- Assessing the Bone-Forming Potential of Pericytes -- Manipulating Pericyte Function with MicroRNAs -- Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals -- Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes -- Metastatic Dissemination Mimicked in a Multicellular Transwell Assay.
520			_aThis volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials of a cell lineage of increasing interest and therapeutic significance. .
700	1		_aPéault, Bruno M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071610558
776	0	8	_iPrinted edition: _z9781071610572
776	0	8	_iPrinted edition: _z9781071610589
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1056-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233476 _d233476