000			03935nam a22003735i 4500
001			233477
003			ES-VaUE
005			20221220020502.0
007			cr nn 008mamaa
008			210212s2021 xxu| s |||| 0|eng d
020			_a9781071611159
024	7		_a10.1007/978-1-0716-1115-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aFluorescence In-Situ Hybridization (FISH) for Microbial Cells _bMethods and Concepts _cedited by Nuno F. Azevedo, Carina Almeida.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 334 páginas) _b52 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2246
505	0		_aAn Introduction to Fluorescence in situ Hybridization in Microorganisms -- FISH Variants -- Bioinformatic Tools and Guidelines for the Design of Fluorescence in situ Hybridization Probes -- FISH in Suspension or in Adherent Cells -- Application of Nucleic Acid Mimics in Fluorescence in situ Hybridization -- Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes -- Characterisation of Social Interactions and Spatial Arrangement of Individual Bacteria in Multi-Strain or Multispecies Biofilm Systems using Nucleic acid mimics-Fluorescence in situ Hybridization -- Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces -- CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples -- Fluorescence in Situ Hybridization with Quantum Dot Labels in E. coli Cells -- Monitoring Bacteriophage Infection on Bacterial Cells using FISH -- Linking Microbes to their Genes at Single Cell Level with direct-geneFISH -- Assigning Function To Phylogeny: FISH-nanoSIMS -- Assigning Function to Phylogeny: MAR-FISH -- Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-based Fluorescence in situ hybridization (fliFISH) -- Integration of FISH and Microfluidics -- Flow-FISH using Nucleic Acid Mimic Probes for the Detection of Bacteria -- FISH in Food Samples -- Extraction of Microbial Cells from Environmental Samples for FISH Approaches -- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology -- Computational Resources and Strategies to Construct Single-Molecule Models of FISH.
520			_aThis volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.
700	1		_aAzevedo, Nuno F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAlmeida, Carina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611142
776	0	8	_iPrinted edition: _z9781071611166
776	0	8	_iPrinted edition: _z9781071611173
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1115-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233477 _d233477