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008			210218s2021 xxu| s |||| 0|eng d
020			_a9781071612460
024	7		_a10.1007/978-1-0716-1246-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNext Generation Culture Platforms for Reliable In Vitro Models _bMethods and Protocols _cedited by Tiziana A.L. Brevini, Alireza Fazeli, Kursad Turksen.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 307 páginas) _b69 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2273
505	0		_aRecreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State -- Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids -- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation -- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors -- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres -- Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models -- Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues -- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models -- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold -- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells -- 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids -- Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics -- Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC) -- Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns -- Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer -- Isolation, Characterization, and MicroRNAs Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids -- Biomimetic 3D-Bone Tissue Model -- Using the Air-Liquid Interface Approach to Foster Apical-Basal Polarization of Mammalian Female Reproductive Tract Epithelia In Vitro -- Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa -- Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro.
520			_aThis detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.
700	1		_aBrevini, Tiziana A.L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFazeli, Alireza _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTurksen, Kursad _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612453
776	0	8	_iPrinted edition: _z9781071612477
776	0	8	_iPrinted edition: _z9781071612484
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1246-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233480 _d233480