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008			210219s2021 xxu| s |||| 0|eng d
020			_a9781071609705
024	7		_a10.1007/978-1-0716-0970-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGermline Development in the Zebrafish _bMethods and Protocols _cedited by Roland Dosch.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIV, 383 páginas) _b87 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2218
505	0		_aSeparation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish -- The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins -- Computer-Assisted Sperm Analysis to Test Environmental Toxicants -- Cryopreservation and Transplantation of Spermatogonial Stem Cells -- Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos -- Chemical Genetics: Manipulating the Germline with Small Molecules -- In vitro Induction of Teleost PGCs -- Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization -- Cryopreservation of Pooled Sperm Samples -- Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery -- In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads -- Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries -- Detection of the Polar Body after Fertilization -- Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles -- Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells -- Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos -- Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy -- Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo -- Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining -- Histological Analysis of Gonads in Zebrafish -- Manipulating and Visualizing the Germline with Transgenic Lines -- Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs -- In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish -- In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation -- The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life -- Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach -- Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos -- -Massively Parallel Analysis of Regulatory RNA Sequences -- Exploring Translational Control of Maternal mRNAs in Zebrafish. .
520			_aThis volume details a wide range of methods, ranging from beginner through advanced, used to further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aDosch, Roland _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071609699
776	0	8	_iPrinted edition: _z9781071609712
776	0	8	_iPrinted edition: _z9781071609729
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0970-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233481 _d233481