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008			210413s2021 xxu| s |||| 0|eng d
020			_a9781071614143
024	7		_a10.1007/978-1-0716-1414-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aYarrowia lipolytica _bMethods and Protocols _cedited by Ian Wheeldon, Mark Blenner.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 250 páginas) _b65 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2307
505	0		_aGenome Engineering of Yarrowia lipolytica with the piggyBac Transposon System -- Genome Editing in Y. lipolytica Using TALENs -- EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica -- Simultaneous gene excision and integration by dual-guide CRISPR-Cas9 -- Gene excision by dual-guide CRISPR-Cas9 -- CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica. -- Implementing CRISPR-Cas12a for Efficient Genome-editing in Yarrowia lipolytica -- Guide RNA Design for Genome-wide CRISPR Screens in Yarrowia lipolytica -- Targeted Integration through Transformation of Hydroxyurea-Arrested Cells -- Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica -- Engineering Yarrowia lipolytica for production of fatty alcohols with YaliBrick Vectors -- Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica -- Pathway Engineering For Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica -- Droplet-based Microfluidic High-throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica -- Yarrowia lipolytica Bioprocess Development - From Flask to Bioreactor -- Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica.
520			_aThis volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.
700	1		_aWheeldon, Ian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBlenner, Mark _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071614136
776	0	8	_iPrinted edition: _z9781071614150
776	0	8	_iPrinted edition: _z9781071614167
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1414-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233486 _d233486