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020			_a9781071611975
024	7		_a10.1007/978-1-0716-1197-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein-Ligand Interactions _bMethods and Applications _cedited by Tina Daviter, Christopher M. Johnson, Stephen H. McLaughlin, Mark A. Williams.
250			_a3rd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 492 páginas) _b123 ilustraciones, 80 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2263
505	0		_aAssessing and Improving Protein Sample Quality -- A Familiar Protein-Ligand Interaction Revisited with Multiple Methods -- Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics -- Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins -- Isothermal Titration Calorimetry -- Measuring the Kd of Protein-Ligand Interactions Using Microscale Thermophoresis -- Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of Complex Protein-Ligand Interactions -- Indirect Detection of Ligand Binding by Thermal Melt Analysis -- The Use of Acoustic Mist Ionization Mass Spectrometry for High-throughput Screening -- Ligand Discovery - High Through-put Binding: Fluorescence Polarization (Anisotropy) -- Fragment Screening by NMR -- A Quick Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for Determining Protein-Nucleotide Affinities -- Measurement of Nucleotide Hydrolysis using Fluorescent Biosensors for Phosphate -- Gel-based Analysis of Protein-Nucleic Acid Interactions -- Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome using SwitchSENSE -- Biolayer Interferometry: Protein-RNA Interactions -- Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip -- Characterization of Protein Nucleic Acid complexes by Size-Exclusion Chromatography Coupled with Light Scattering, Absorbance and Refractive Index Detectors -- Analytical Ultracentrifugation for Analysis of Protein Nucleic Acid Interactions -- Studying RNA-Protein Complexes Using X-ray Crystallography -- Flow Linear Dichroism of Protein-membrane Systems -- Probing Protein-Membrane Interactions and Dynamics Using Hydrogen-Deuterium eXchange Mass Spectrometry (HDX-MS).
520			_aThis third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.
700	1		_aDaviter, Tina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aJohnson, Christopher M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMcLaughlin, Stephen H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWilliams, Mark A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611968
776	0	8	_iPrinted edition: _z9781071611982
776	0	8	_iPrinted edition: _z9781071611999
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1197-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233488 _d233488