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020 _a9781071611340
024 7 _a10.1007/978-1-0716-1134-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Transposable Elements
_bMethods and Protocols
_cedited by Jungnam Cho.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XV, 272 páginas)
_b61 ilustraciones, 46 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2250
505 0 _aA Predictive Approach to Infer the Activity and Natural Variation of Retrotransposon Families in Plants -- In silico Identification of Stress Associated Transposable Elements in Arabidopsis thaliana using Public Transcriptome Data -- A Practical Guide on Computational Tools and Databases for Transposable Elements in Plants -- A Tutorial of EDTA: Extensive de-novo TE Annotator -- Mining of Miniature Transposable Elements in Brassica species at BrassicaTED -- Genome-wide Identification of Miniature Inverted-Repeat Transposable Elements by Targeted High-Throughput Sequencing -- Identification of Extrachromosomal Circular Forms of Active Transposable Elements Using Mobilome-Seq -- Identification of Active Transposable Elements in Plants: The mobilome-seq Approach -- High-throughput Profiling of Extrachromosomal Linear DNAs of Long Terminal Repeat Retrotransposons by ALE-seq -- Bioinformatics Analysis Guides to LTR Retrotransposon-Derived Extrachromosomal Linear DNAs Identified by ALE-seq -- Determination of TE insertion Positions using Transposon Display -- Detection of Transposition Events from Next-Generation Sequencing Data -- Rapid Detection of Transposon-Induced Genome Rearrangements -- Detection of Transposable Element Insertions in Arabidopsis Using Sequence Capture -- Efficient Detection of Transposable Element Insertion Polymorphisms Between Genomes Using Short-Read Sequencing Data -- Quantitative Measurement of Transposon Copy Number using the Droplet Digital PCRDetecting Signatures of TE Polymorphisms in Short-read Sequencing Data -- How to Activate Heat-responsible Retrotransposon ONSEN in Brassicaceae Species -- Measurement of Genetic Mobility Using a Transposon-Based Marker System In Sorghum -- Sequence-specific Amplified Polymorphism (SSAP) and Sequence Characterized Amplified Region (SCAR) Markers in Zea mays -- Analysis of Plant DNA Methylation Profiles using R -- Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Seed Tissue Genomic DNA -- Transposon-Based Tagging in Silico using FastPCR Software -- Bamboo Transposon Research: Current Status and Perspectives. .
520 _aThis volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.
700 1 _aCho, Jungnam
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071611333
776 0 8 _iPrinted edition:
_z9781071611357
776 0 8 _iPrinted edition:
_z9781071611364
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1134-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233491
_d233491