000			04299nam a22003735i 4500
001			233503
003			ES-VaUE
005			20221220020504.0
007			cr nn 008mamaa
008			210519s2021 xxu| s |||| 0|eng d
020			_a9781071612941
024	7		_a10.1007/978-1-0716-1294-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTET Proteins and DNA Demethylation _bMethods and Protocols _cedited by Ozren Bogdanovic, Michiel Vermeulen.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 320 páginas) _b56 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2272
505	0		_aReduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine -- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine -- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS) -- ELISA-Based Quantitation of Global 5hmC Levels -- Avidin-Biotin ELISA-Based Detection of 5hmC -- Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS -- Distinguishing Active Versus Passive DNA Demethylation using Illumina MethylationEPIC BeadChip Microarrays -- Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions -- TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling -- Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9 -- High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation -- Identifying Protein-(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics -- Purification of TET Proteins -- Uncovering Sequence Specific Transcription Factors Interacting with TET2 -- ChIP-Sequencing of TET Proteins -- Harnessing Alternative Substrates to Probe TET Family Enzymes -- Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts.
520			_aThis volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry approaches. Part Two looks at data analyses protocols for distinguishing acting versus passive DNA demethylation and estimation of 5mC and 5hmC levels. Part Three deals with a new topic that takes advantage of modified CRISPR/Cas9 genome editing systems to target DNA demethylation activity to genomic loci of interest. Part Four discusses protocols that detail how to purify TET proteins and unravel their protein interactions, and Part Five looks at the assessment of TET protein function and activity in vivo and in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, TET Proteins and DNA Demethylation: Methods and Protocols is a valuable resource that aims to help research scientists at all levels working in the fields of DNA demethylation dynamics. Chapters 3, 7 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aBogdanovic, Ozren _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aVermeulen, Michiel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071612934
776	0	8	_iPrinted edition: _z9781071612958
776	0	8	_iPrinted edition: _z9781071612965
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1294-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233503 _d233503