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008			210520s2021 xxu| s |||| 0|eng d
020			_a9781071611661
024	7		_a10.1007/978-1-0716-1166-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPDZ Mediated Interactions _bMethods and Protocols _cedited by Jean-Paul Borg.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 292 páginas) _b100 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2256
505	0		_aIdentification of PDZ Interactions by Yeast Two-Hybrid -- Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis -- Identification of PDZ Interactions by Proteomic Peptide Phage Display -- A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay -- Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore -- PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations -- Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module -- A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics -- Unveiling the Folding Mechanism of PDZ Domains -- Development of Peptide-Based PDZ Domain Inhibitors -- Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs -- Chemical Synthesis of PDZ Domains -- Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains -- Computational Design of PDZ:peptide Binding -- Mechanoregulation of PDZ Proteins, An Emerging Function -- Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains.
520			_aThis volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field. .
700	1		_aBorg, Jean-Paul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071611654
776	0	8	_iPrinted edition: _z9781071611678
776	0	8	_iPrinted edition: _z9781071611685
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1166-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233504 _d233504