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008			210730s2021 xxu| s |||| 0|eng d
020			_a9781071614808
024	7		_a10.1007/978-1-0716-1480-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCardiovascular Development _bMethods and Protocols _cedited by Xu Peng, Warren E. Zimmer.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 162 páginas) _b37 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2319
505	0		_aTissue clearing and 3-D visualization of Vasculature with the PEGASOS Method -- Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample -- RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells -- Preparation of Neonatal Rat Papillary Muscles for Contractile Studies -- In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine -- Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells -- Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining -- Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue -- Laser-induced Choroidal Neovascularization in Rats -- Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain -- Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy -- Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues -- Visualization of Fundus Blood Vessels -- Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements -- Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery -- Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus -- Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.
520			_aThis second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.
700	1		_aPeng, Xu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aZimmer, Warren E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071614792
776	0	8	_iPrinted edition: _z9781071614815
776	0	8	_iPrinted edition: _z9781071614822
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1480-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233511 _d233511