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020			_a9781071615973
024	7		_a10.1007/978-1-0716-1597-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEnhancers and Promoters _bMethods and Protocols _cedited by Tilman Borggrefe, Benedetto Daniele Giaimo.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 376 páginas) _b69 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2351
505	0		_aA New Toolbox to Analyze Enhancer-Promoter Functions -- Global Run-on Sequencing (GRO-Seq) -- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq) -- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters -- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq -- Measuring Chromatin Accessibility: ATAC-Seq -- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles -- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes -- Low Input Targeted Chromatin Capture (low-T2C) -- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq) -- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies -- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions -- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C) -- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions -- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin -- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq) -- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators -- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors -- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing -- Low Input Whole-Genome Bisulfite Sequencing.
520			_aThis volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting. Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments. Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
700	1		_aBorggrefe, Tilman _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGiaimo, Benedetto Daniele _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071615966
776	0	8	_iPrinted edition: _z9781071615980
776	0	8	_iPrinted edition: _z9781071615997
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1597-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233515 _d233515