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020 _a9781071616178
024 7 _a10.1007/978-1-0716-1617-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPeptide Conjugation
_bMethods and Protocols
_cedited by Waleed M. Hussein, Rachel J. Stephenson, Istvan Toth.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XII, 324 páginas)
_b79 ilustraciones, 59 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2355
505 0 _aPeptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition -- Conjugation of Peptides to Gold Nanoparticles -- Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus -- Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides -- Modification of Nanoparticles with Transferrin for Targeting Brain Tissues -- Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition -- Vitamin B12 - Peptide Nucleic Acid Conjugates -- Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides -- Solid-State, Thermal Synthesis of Peptide/Protein-Boron Cluster Conjugates -- Bioconjugation of Peptides to Hybrid Gold Nanoparticles -- Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2 -- Electrochemically-Enabled C-Terminal Peptide Modifications -- Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates -- Chemical Protein Synthesis by Chemoselective α-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline -- Site-Specific Modification of Single-Chain Affinity Ligands for Fluorescence Labeling, Radiolabeling, and Bioconjugation -- Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags -- The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library -- The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation -- Synthesis of Lipopeptides by CLipPA Chemistry -- Constraining TAT Peptide by γPNA Hairpin for Enhanced Cellular Delivery of Biomolecules -- Preparation of mRNA Polyplexes with Post-Conjugated Endosome-Disruptive Peptides -- Targeted Subcellular Protein Delivery Using Cleavable Cyclic Cell-Penetrating Peptide-Conjugates -- Facile Chemoselective Modification of Thio-Ethers Generates Chiral Center-Induced Helical Peptides.
520 _aThis volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Peptide Conjugation: Methods and Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. .
700 1 _aHussein, Waleed M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aStephenson, Rachel J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aToth, Istvan
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071616161
776 0 8 _iPrinted edition:
_z9781071616185
776 0 8 _iPrinted edition:
_z9781071616192
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1617-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233516
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