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008			210929s2021 xxu| s |||| 0|eng d
020			_a9781071616215
024	7		_a10.1007/978-1-0716-1621-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Persistence _bMethods and Protocols _cedited by Natalie Verstraeten, Jan Michiels.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 292 páginas) _b51 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2357
505	0		_aStudying Bacterial Persistence: Established Methods And Current Advance -- Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases -- A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters -- Enrichment of Persister Cells through β-lactam-induced Filamentation and Size Separation -- Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies -- Observing Bacterial Persistence at Single-Cell Resolution -- Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis -- Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation -- Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence -- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods -- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa -- The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment -- Detecting persister awakening determinants -- Monitoring Persister Resuscitation with Flow Cytometry -- Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters -- In vitro Models for the Study of the Intracellular Activity Of Antibiotics -- Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis -- Studying Antibiotic Persistence During Infection. [NV1].
520			_aThis volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.
700	1		_aVerstraeten, Natalie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMichiels, Jan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071616208
776	0	8	_iPrinted edition: _z9781071616222
776	0	8	_iPrinted edition: _z9781071616239
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1621-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233520 _d233520