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020 _a9781592599363
024 7 _a10.1385/1592599362
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aLymphoma
_cedited by Tim Illidge, Peter W. M. Johnson.
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XVIII, 342 páginas)
_b43 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v115
505 0 _aPurification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins -- Molecular Diagnosis of Lymphoma -- Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cytometry -- Karyotyping Lymph Node Biopsies in Non-Hodgkin's Lymphoma -- Identification of Lymphoma-Associated Antigens Using SEREX -- Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia -- Idiotype Gene Rescue in Follicular Lymphoma -- Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma -- T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma -- Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain Reaction -- Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic Leukemia -- Splenic Marginal Zone Lymphoma -- BCL-6 -- Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL -- Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5?RACE -- Follicular Lymphoma.
520 _aThe tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas.
700 1 _aIllidge, Tim
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aJohnson, Peter W. M
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373954
776 0 8 _iPrinted edition:
_z9781588291592
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599362
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2005
999 _c233551
_d233551