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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592814
024	7		_a10.1385/1592592813 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGTPase Protocols _bThe Ras Superfamily _cedited by Ed Manser, Thomas Leung.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XI, 267 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v189
505	0		_aGeneral Protocols -- The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases -- Preparation of GTPases for Structural and Biophysical Analysis -- Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes -- Fluorescence Methods in the Study of Small GTP-Binding Proteins -- Specific Methods -- Ras and Rac as Activators of Reactive Oxygen Species (ROS) -- Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs -- A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays -- Determination of the Activity of Rho-Like GTPases in Cells -- of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases -- Effects of Rho Family GTPases on Cell-Cell Adhesion -- Cell Motility and Invasion Assays -- Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases -- Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase -- Preparation of Myristoylated Arf1 and Arf6 Proteins -- ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins -- Arf6 and its Role in Cytoskeletal Modulation -- ARF GTPase-Activating Protein 1 -- The Use of Permeabilized Cell Systems to Study Nuclear Transport -- Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes -- Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton.
520			_aSmall GTPase binding proteins (GTPases) are an ancient group of proteins that play key roles in almost every aspect of cell biology, from cell proliferation to nuclear transport. In GTPase Protocols: The Ras Superfamily, Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.
700	1		_aManser, Ed _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLeung, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372612
776	0	8	_iPrinted edition: _z9780896039346
776	0	8	_iPrinted edition: _z9781489938749
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592813 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c233554 _d233554