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020			_a9781592590568
024	7		_a10.1385/159259056X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aComplement Methods and Protocols _cedited by B. Paul Morgan.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (IX, 259 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v150
505	0		_aThe Complement System:An Overview -- Purification of Complement Components, Regulators, and Receptors by Classical Methods -- Immunoaffinity Methods for Purification of Complement Components and Regulators -- Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates -- Measurement of Complement Lysis of Nucleated Cells -- Functional Assays for Complement Regulators -- Immunochemical Measurement of Complement Components and Activation Products -- Complement Deposition in Tissues -- Complement Regulators and Receptors in Tissues -- Measurement of C3 Fragment Deposition on Cells -- Screening for Complement Deficiency -- C1-Inhibitor: Antigenic and Functional Analysis -- Autoantibodies to Complement Components -- Allotyping of Complement Components -- Complement and Immune Complexes -- Knocking Out Complement Genes -- Inherited Complement Deficiencies in Animals.
520			_aAlthough the complement system-a vital part of the body's defense against bacteria-was discovered more than a century ago, its study has undergone a renaissance with the identification of its regulatory molecules and the realization that these molecules can be used therapeutically. In Complement Methods and Protocols, B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of complement function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.
700	1		_aMorgan, B. Paul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371165
776	0	8	_iPrinted edition: _z9780896036543
776	0	8	_iPrinted edition: _z9781489941626
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259056X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c233555 _d233555