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008			151020s2016 xxu| s |||| 0|eng d
020			_a9781493931583
024	7		_a10.1007/978-1-4939-3158-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Genetic Manipulation of Staphylococci _bMethods and Protocols _cedited by Jeffrey L. Bose.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 186 páginas) _b23 ilustraciones, 7 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1373
505	0		_aUnderstanding Staphylococcal Nomenclature -- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus -- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci -- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning -- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products -- Method for Preparation and Electroporation of S. aureus and S. epidermidis -- Rapid Isolation of DNA from Staphylococcus -- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method -- Bacteriophage Transduction in Staphylococcus aureus -- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus -- Conjugative Transfer in Staphylococcus aureus -- Allelic Exchange -- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917 -- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis -- Chemical and UV Mutagenesis -- Pulsed Field Gel Electrophoresis -- RNA-Sequencing of Staphylococcus aureus Messenger RNA -- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression -- Electrophoretic Mobility Shift Assays -- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.
520			_aThis detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.
700	1		_aBose, Jeffrey L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493931576
776	0	8	_iPrinted edition: _z9781493931590
776	0	8	_iPrinted edition: _z9781493949007
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3158-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233556 _d233556