000			04323nam a22003855i 4500
001			233560
003			ES-VaUE
005			20221220020507.0
007			cr nn 008mamaa
008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592593996
024	7		_a10.1385/1592593992 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aVaccine Protocols _cedited by Andrew P. Robinson, Martin P. Cranage, Michael J. Hudson.
250			_a2nd edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 416 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v87
505	0		_aOverview of Vaccines -- Temperature-Sensitive Mutant Vaccines -- Live Viral Vectors -- Live Viral Vectors -- Live Viral Vectors -- Development of Attenuated Salmonella Strains That Express Heterologous Antigens -- Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria -- Synthetic Peptides -- Genetic Detoxification of Bacterial Toxins -- Preparation of Polysaccharide-Conjugate Vaccines -- Adjuvant Formulations for Experimental Vaccines -- Incorporation of Immunomodulators into Plasmid DNA Vaccines -- Microencapsulation of Vaccine Antigens -- Lyophilization of Vaccines -- Stimulation of Mucosal Immunity -- Induction and Detection of T-Cell Responses -- Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses -- Assessment of Functional Antibody Responses -- The Use of Complete Genome Sequences in Vaccine Design -- Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment -- Clinical Trials -- Assuring the Quality and Safety of Vaccines -- DNA Vaccination -- From Vaccine Research to Manufacture.
520			_aThis extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.
700	1		_aRobinson, Andrew P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCranage, Martin P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHudson, Michael J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373824
776	0	8	_iPrinted edition: _z9781588291400
776	0	8	_iPrinted edition: _z9781489938251
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593992 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2003
999			_c233560 _d233560