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008			180112s2018 xxu| s |||| 0|eng d
020			_a9781493976652
024	7		_a10.1007/978-1-4939-7665-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHypoxia _bMethods and Protocols _cedited by L. Eric Huang.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 302 páginas) _b73 ilustraciones, 63 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1742
505	0		_aWhole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma -- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina Bead Arrays -- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens -- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens -- Isolation of GBM Stem Cells with Flow Cytometry -- Lentiviral Transduction of Primary Human Glioblastoma Cultures -- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors -- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF -- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro -- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry -- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS) -- Single-Cell RNA Sequencing of Glioblastoma Cells -- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures -- Orthotopic Patient-derived Glioblastoma Xenografts in Mice -- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice -- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy -- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection -- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma -- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. .
520			_aThis volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond.
700	1		_aHuang, L. Eric _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493976645
776	0	8	_iPrinted edition: _z9781493976669
776	0	8	_iPrinted edition: _z9781493985388
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7665-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233564 _d233564