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001			233571
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008			180501s2018 xxu| s |||| 0|eng d
020			_a9781493977789
024	7		_a10.1007/978-1-4939-7778-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDigital PCR _bMethods and Protocols _cedited by George Karlin-Neumann, Francisco Bizouarn.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 557 páginas) _b141 ilustraciones, 128 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1768
505	0		_aEntering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR -- Basic Concepts and Validation of Digital PCR Measurements -- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean? -- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma -- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events -- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples -- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR -- Testing of General and Human-Associated Fecal Contamination in Waters -- Analyzing Copy Number Variation with Droplet Digital PCR -- Assessing HER2 Amplification in Plasma cfDNA -- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants -- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR -- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients -- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders -- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio™ 3D Digitial PCR System -- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR -- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA -- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping -- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set -- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR -- DNA Methylation Analysis Using Droplet Digital PCR -- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR -- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression -- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR™ Assays -- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry -- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141 -- Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay -- Phasing DNA Markers using Digital PCR -- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity -- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.
520			_aThis volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.".
700	1		_aKarlin-Neumann, George _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBizouarn, Francisco _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493977765
776	0	8	_iPrinted edition: _z9781493977772
776	0	8	_iPrinted edition: _z9781493992829
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7778-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233571 _d233571