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008			210413s2021 xxu| s |||| 0|eng d
020			_a9781071610169
024	7		_a10.1007/978-1-0716-1016-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Complement System _bInnovative Diagnostic and Research Protocols _cedited by Lubka T. Roumenina.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 267 páginas) _b55 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2227
505	0		_aThe Benefits of Complement Measurements for the Clinical Practice -- Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source -- Quantification of Complement Proteins with Special Reference to C1q: Multiplex vs ELISA vs Rocket Immunoelectrophoresis vs Nephelometry -- C3dg Quantification by PEG-precipitation and ELISA or TRIFMA -- Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope Based Enzyme-Linked Immunosorbent Assay -- Sheep Erythrocytes Preparation for Hemolytic Tests Exploring Complement Functional Activities -- Hemolytic Tests Exploring Factor H Functional Activities -- Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity -- Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry -- Anti-C1q Auto-Antibodies: Standard Quantification and Innovative ELISA -- Anti-C1-Inhibitor Auto-Antibodies Detection by ELISA -- Anti-ficolin-2 and anti-ficolin-3 Autoantibodies Detection by ELISA -- Detection of anti-C3b Autoantibodies by ELISA -- Detection of Complement Factor B Autoantibodies by ELISA -- Detection of C3 Nephritic Factor by Hemolytic Assay -- Detection of Genetic Rearrangements inthe Regulators of Complement Activation RCA Cluster by High Throughput Sequencing and MLPA -- Complement Detection in Mouse Kidneys by Immunofluorescence -- Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence -- Analysis Of The Ligand Recognition Specificities Of Human Ficolins Using Surface Plasmon Resonance -- Methods for Assessment of Interactions of Proteins with Heme - Application for Complement Proteins and Immunoglobulins -- Evaluation of Binding Kinetics and Thermodynamics of Antibody-antigen Interactions and Interactions Involving Complement Proteins -- Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications. .
520			_aThis volume describes a range of methods to be used in complement laboratories use and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aRoumenina, Lubka T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071610152
776	0	8	_iPrinted edition: _z9781071610176
776	0	8	_iPrinted edition: _z9781071610183
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1016-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233574 _d233574