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001			233577
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008			210527s2021 xxu| s |||| 0|eng d
020			_a9781071614952
024	7		_a10.1007/978-1-0716-1495-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aExperimental Models of Parkinson's Disease _cedited by Yuzuru Imai.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 217 páginas) _b57 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2322
505	0		_aα-Synuclein Seeding Assay Using RT-QuIC -- Electron-Microscopic Analysis of α-Synuclein Fibrils -- α-Synuclein Seeding Assay Using Cultured Cells -- Analysis of α-Synuclein in Exosomes -- Measurement of GCase Activity in Cultured Cells -- Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells -- Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement -- Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells -- Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells -- Generation of Mitochondrial Toxin Rodent Models of Parkinson's Disease Using 6-OHDA, MPTP, and Rotenone -- Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease -- α-Synuclein Propagation Mouse Models of Parkinson's Disease -- Common Marmoset Model of α-Synuclein Propagation -- Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections -- Deep Brain Stimulation Using Animal Models of Parkinson's Disease -- Assessment of Cytotoxicity of α-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy -- The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons -- Analysis of Dopaminergic Functions in Drosophila -- Evaluation of Mitochondrial Function and Morphology in Drosophila -- Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons.
520			_aThis detailed book focuses on cutting-edge methods for Parkinson's disease (PD) research, such as the analysis of the prion-like properties of α-synuclein, mitochondrial functions related to the PINK1-Parkin pathway/CHCHD2, the endolysosome pathway related to LRRK2, VPS35, and ATP13A2 using cultured cells (including patient iPS cells), deep brain stimulation therapy, classic mitochondrial toxins related to PD, and genetic associations and screenings using mammalian and invertebrate genetic models of PD. The collection intends to serve as an introductory protocol book for basic research on PD pathogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Experimental Models of Parkinson's Disease is an ideal guide for researchers continuing to work toward making PD risk predictable and surmountable.
700	1		_aImai, Yuzuru _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071614945
776	0	8	_iPrinted edition: _z9781071614969
776	0	8	_iPrinted edition: _z9781071614976
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1495-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233577 _d233577