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008			210530s2021 xxu| s |||| 0|eng d
020			_a9781071615072
024	7		_a10.1007/978-1-0716-1507-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCytotoxic T-Cells _bMethods and Protocols _cedited by Margherita Gigante, Elena Ranieri.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 247 páginas) _b29 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2325
505	0		_aFlow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting -- Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells -- Tumor Infiltrating T-Cell Cytotoxicity Assay -- Antigen-Specific In Vivo Killing Assay -- CTL ELISPOT Assay and T-Cell Detection -- Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single Cell-RNA Sequencing -- In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells -- Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray -- A Review of Proteomics Strategies to Study T-Cells Activation and Function in Cancer Disease -- Methods to Study Post-Translational Modification Patterns in Cytotoxic T-Cells and Cancer -- Whole Exome Sequencing for the Identification of Mutations in Cd8+T-Cells -- Handling High-Throughput Omics Data for Systems Genetics Analysis -- Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System -- Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview -- Protein-Bound Uremic Toxins and Immunity -- Gut Microbiota, the Immune System, and Cytotoxic T Lymphocytes.
520			_aThis new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with in vitro and in vivo cytotoxicity assays, methods to detect senescent T cells, in vivo and in vitro models to understand immune and bone cells cross-talk, microscopy and in vivo imaging, as well as "Omics" approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and key implementation advice for best results in the lab. Authoritative and up-to-date, Cytotoxic T-Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers working with these vital cells.
700	1		_aGigante, Margherita _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRanieri, Elena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071615065
776	0	8	_iPrinted edition: _z9781071615089
776	0	8	_iPrinted edition: _z9781071615096
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1507-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233581 _d233581