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008			210606s2021 xxu| s |||| 0|eng d
020			_a9781071614334
024	7		_a10.1007/978-1-0716-1433-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMitochondrial Regulation _bMethods and Protocols _cedited by Carlos M. Palmeira, Anabela P. Rolo.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 318 páginas) _b64 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2310
505	0		_aIsolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression -- Determination of Oxidative Phosphorylation Complexes Activities -- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function -- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs) -- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes -- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections -- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies -- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species -- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases -- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria -- Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry -- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD) -- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria -- Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis -- Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content -- Following the Dynamism of the Mitochondrial Network in T Cells -- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.
520			_aThis fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.
700	1		_aPalmeira, Carlos M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRolo, Anabela P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071614327
776	0	8	_iPrinted edition: _z9781071614341
776	0	8	_iPrinted edition: _z9781071614358
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1433-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233588 _d233588