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008			210624s2021 xxu| s |||| 0|eng d
020			_a9781071615034
024	7		_a10.1007/978-1-0716-1503-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPseudogenes _bFunctions and Protocols _cedited by Laura Poliseno.
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 384 páginas) _b78 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2324
505	0		_aPseudogenes: Four Decades of Discovery -- Methods to Identify and Study the Evolution of Pseudogenes using a Phylogenetic Approach -- Computational Methods For Pseudogene Annotation Based on Sequence Homology -- A Methodology to Study pseudogenized lincRNAs -- GENCODE Pseudogenes -- Comprehensive Detection of Pseudogenes Transcribed by Readthrough -- Mathematical modeling of ceRNA-based interactions -- Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation -- Pseudogenes as Competitive Endogenous RNAs: Testing miRNA Dependency -- An Overview of the Computational Models Dealing with the Regulatory ceRNA Mechanism and ceRNA Deregulation in Cancer -- Studying the Oncosuppressive Functions of PTENP1 as a ceRNA -- Methods to Study Protein-Binding to Pseudogene Transcript -- Chromatin Regulation at Parental Gene Promoters by Pseudogene Sense lncRNAs -- Pseudogenes: A Novel Source of Trans Acting Antisense RNAs -- Methods to Study Translated Pseudogenes: Recombinant Expression and Complementation, Targeted Proteomics and RNA Profiling -- A Restriction Endonuclease-Based Assay to distinguish NANOGP8 Retrogene from Parental NANOG -- CRISPR/Cas Technologies Applied to Pseudogenes -- Strategies to Study the Functions of Pseudogenes in Mouse Models of Cancer -- Pseudogene Profiling For Cancer Subtype Classification -- Pseudogenes as Biomarkers and Therapeutic Targets in Human Cancers -- Methods for the Detection of Circulating Pseudogenes and their use as Cancer Biomarker -- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.
520			_aThis second edition explores techniques involving pseudogenic DNA, RNA, and proteins. Chapters describe how to identify pseudogenes and how to study the functions and alterations of pseudogenic RNA and proteins, both in vitro, and in vivo. Written in the highly successful Methods in Molecular Biology series format, methodological chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting while avoiding known pitfalls. Authoritative and cutting-edge, Pseudogenes: Functions and Protocols, Second Edition aims to be a practical guide to researches, useful to help further their study in this field.
700	1		_aPoliseno, Laura _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071615027
776	0	8	_iPrinted edition: _z9781071615041
776	0	8	_iPrinted edition: _z9781071615058
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1503-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2021
999			_c233595 _d233595