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020 _a9781071616451
024 7 _a10.1007/978-1-0716-1645-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Circular RNAs
_bMethods and Protocols
_cedited by Luis María Vaschetto.
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (X, 196 páginas)
_b43 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2362
505 0 _aIdentification and Characterization of Plant Circular RNAs -- Experimental Strategies for Studying the Function of Plant circRNAs -- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization -- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development -- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing -- Identification and Functional Characterization of Viroid Circular RNAs -- Circular RNA Databases -- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs -- Computational Analysis of Transposable Elements and circRNAs in Plants -- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database -- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.
520 _aThis volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field. .
700 1 _aVaschetto, Luis María
_eeditor literario
_0(orcid)0000-0002-9290-4335
_1https://orcid.org/0000-0002-9290-4335
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071616444
776 0 8 _iPrinted edition:
_z9781071616468
776 0 8 _iPrinted edition:
_z9781071616475
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1645-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2021
999 _c233596
_d233596