000			03517nam a22003615i 4500
001			233652
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007			cr nn 008mamaa
008			180201s2018 xxu| s |||| 0|eng d
020			_a9781493976522
024	7		_a10.1007/978-1-4939-7652-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aExtracellular RNA _bMethods and Protocols _cedited by Tushar Patel.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 216 páginas) _b43 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1740
505	0		_aExtracellular RNAs - A New Awareness of Old Perspectives -- Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles -- Extracellular RNA Isolation from Cell Culture Supernatant -- Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture -- Isolation of Extracellular RNA from Serum/Plasma -- Isolation of Extracellular RNA from Bile -- Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes -- Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids -- Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography -- Detection and Analysis of Non-vesicular Extracellular RNA -- Isolation of Plasma Lipoproteins as a Source of Extracellular RNA -- Droplet Digital PCR for Quantitation of Extracellular RNA -- Preparation of Small RNA NGS Libraries from Biofluids -- Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString -- Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs -- Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs. .
520			_aThis volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
700	1		_aPatel, Tushar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493976515
776	0	8	_iPrinted edition: _z9781493976539
776	0	8	_iPrinted edition: _z9781493985357
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7652-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233652 _d233652