000			04994nam a22003735i 4500
001			233655
003			ES-VaUE
005			20221220020513.0
007			cr nn 008mamaa
008			180509s2018 xxu| s |||| 0|eng d
020			_a9781493978113
024	7		_a10.1007/978-1-4939-7811-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPeptide Self-Assembly _bMethods and Protocols _cedited by Bradley L. Nilsson, Todd M. Doran.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 452 páginas) _b130 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1777
505	0		_aMethods for Structural Analysis of Aggregates Formed by Self-Assembling Peptides -- Solid state NMR Structura -- Characterization of Self-assembled Peptides With Selective 13C and 15N Isotopic LabelsATR-FTIR Analysis of Amyloid Proteins -- Imaging Protein Fibers at the Nanoscale and In Situ -- Replica Exchange Molecular Dynamics: A Practical Application Protocol with Solutions to Common Problems and a Peptide Aggregation and Self-Assembly Example -- An Aggregate Weight-normalized thioflavin-T Measurement Scale For Characterizing Polymorphic Amyloids And Assembly Intermediates -- Nanoparticle Tracking for Protein Aggregation Research -- Peptide Self-assembly Measured using Fluorescence Correlation Spectroscopy -- A General Method to Prepare Peptide-based Supramolecular Hydrogels -- Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks -- Synthesis of Mikto-Arm Star Peptide Conjugates -- Synthesis and Evaluation of Self-Assembled Nanostructures of Peptide-π chromophore conjugates -- Programmable Fabrication of Multi-layer Collagen Nanosheets of Defined Composition -- Practical Considerations in the Design and use of Immunologically Active Fibrillar Peptide Assemblies -- Microwave-assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines -- Preparation and Screening of Catalytic Amyloid Assemblies.-Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery -- Biogelx: Cell Culture on Gels based on Aromatic Peptide Amphiphiles -- Production and use of Recombinant Aβ for Aggregation Studies -- Disaggregation of Ab42 for Structural and Biochemical Studies -- Preparation of Stable Amyloid-β Oligomers Without Perturbative Methods -- Discriminating Strains of Self-Propagating Protein Aggregates Using a Conformational Stability Assay -- Model Phospholipid Liposomes to Study the b-Amyloid-Peptide-Induced Membrane Disruption -- Using Molecular Tweezers to Remodel Abnormal Protein Self-assembly and Inhibit the Toxicity of Amyloidogenic Proteins -- Incorporation of an azobenzene b-turn Peptidomimetic into Amyloid-b to Probe Potential Structural Motifs Leading to b-sheet self-assembly -- Solid State NMR Studies of Amyloid Materials: A Protocol to Define an Atomic Model of Aβ(1-42) in Amyloid Fibrils -- Experimental and Computational Protocols for Studies of Cross-seeding Amyloid Assemblies.
520			_aThis volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Drug Discovery and Design aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.
700	1		_aNilsson, Bradley L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDoran, Todd M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978090
776	0	8	_iPrinted edition: _z9781493978106
776	0	8	_iPrinted edition: _z9781493992928
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7811-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233655 _d233655