000			04003nam a22003615i 4500
001			233658
003			ES-VaUE
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007			cr nn 008mamaa
008			180514s2018 xxu| s |||| 0|eng d
020			_a9781493978373
024	7		_a10.1007/978-1-4939-7837-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMacrophages _bMethods and Protocols _cedited by Germain Rousselet.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 278 páginas) _b43 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1784
505	0		_aCulture of Human Monocyte-Derived Macrophages -- A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages -- Growing Murine Bone Marrow-Derived Macrophages -- Immortalization of Murine Bone Marrow-Derived Macrophages -- Isolation and Identification of Murine Serous Cavity Macrophages -- Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies -- Isolation and Phenotyping of Adult Mouse Microglial Cells -- Isolation and Phenotyping of Bone Marrow Macrophages -- Isolation and Phenotyping of Intestinal Macrophages -- Phenoytyping Tumor Associated Macrophages -- Activating Murine Macrophages In Vitro -- Polarizing Macrophages In Vitro -- Viral Replication Assay in Bone Marrow-Derived Macrophages -- Macrophage Bactericidal Assays -- Quantitative Phagocytosis Assays in Primary and Cultured Macrophages -- Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM) -- Chromatine Immunoprecipitation in Macrophages -- Transfecting Macrophages -- In Vitro Migration Assays -- Cytokine-Induced Acute Inflammatory Monarticular Arthritis -- Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections -- Genetic Models of Macrophage Depletion -- Depleting Macrophages In Vivo with Clodronate-Liposomes -- Cre Driver Mice Targeting Macrophages.
520			_aThis volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level. The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.
700	1		_aRousselet, Germain _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978366
776	0	8	_iPrinted edition: _z9781493978380
776	0	8	_iPrinted edition: _z9781493993000
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7837-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233658 _d233658