000			03461nam a22003495i 4500
001			233746
003			ES-VaUE
005			20221220020519.0
007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592592630
024	7		_a10.1385/1592592635 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEicosanoid Protocols _cedited by Elias A Lianos.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XI, 296 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v120
505	0		_aUse of a Retroviral Vector for Expression of a Foreign Gene -- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues -- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization -- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin -- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography -- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry -- Assessment of Cyclooxygenase Protein Expression by Western Blotting -- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells -- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction -- Enzyme immunoassays (EIA) of Eicosanoids -- Transcellular Regulation of Eicosanoid Biosynthesis -- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry -- Epoxyeicosatrienoic Acids -- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts -- Assessment of 5-Lipoxygenase Activity and Cellular Distribution -- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids -- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition -- Novel Eicosanoids.
520			_aIn Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
700	1		_aLianos, Elias A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943620
776	0	8	_iPrinted edition: _z9780896036673
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592635 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c233746 _d233746