000			03730nam a22003615i 4500
001			233752
003			ES-VaUE
005			20221220020519.0
007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592596003
024	7		_a10.1385/0896034992 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aClinical Applications of PCR _cedited by Y. M. Dennis Lo.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (X, 353 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v16
505	0		_ato the Polymerase Chain Reaction -- Setting Up a PCR Laboratory -- General Methodology -- Amplification from Archival Materials -- Quantitative PCR -- Mutation Screening Using PCR-SSCP -- Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling -- The Amplification Refractory Mutation System -- Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis -- Long Range PCR -- Generation of Labeled Probes by PCR -- PCR for the Detection of Minority DNA Populations -- In Situ Amplification -- Sequencing of PCR Products -- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) -- Clinical Application -- PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation -- Diagnosis of Mitochondrial Disorders Using the PCR -- PCR Analysis of CD44 Variants in Tumors -- Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction -- Detection of Circulating Tumor Cells Using lmmunobead-PCR -- Single Cell PCR -- PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation -- Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood -- Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescent PCR -- Detection of the Hepatitis C Virus by RT-PCR -- The Application of PCR to the Detection of M. tuberculosis in Sputum Samples -- Microsatellite Analysis in Human Disease.
520			_aClinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The methods described in Clinical Applications of PCR will well serve diverse clinical specialties ranging from hematology/oncology, human genetics, and microbiology, to virology, pathology, and infectious diseases. The book repeatedly demonstrates the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.
700	1		_aLo, Y. M. Dennis _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943514
776	0	8	_iPrinted edition: _z9780896034990
776	0	8	_iPrinted edition: _z9780896033597
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034992 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c233752 _d233752