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001			233753
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007			cr nn 008mamaa
008			161116s2017 xxu| s |||| 0|eng d
020			_a9781493966301
024	7		_a10.1007/978-1-4939-6630-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHistones _bMethods and Protocols _cedited by Benoit Guillemette, Luc R. Gaudreau.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 279 páginas) _b36 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1528
505	0		_aIn Vitro Assembly of Nucleosomes For Binding/Remodeling Assays -- An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays -- Histone Purification from Saccharomyces Cerevisiae -- Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, Hplc-Purified Histones -- Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples -- Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies -- Production and Purification of Antibodies against Histone Modifications -- Immunofluorescence of Histone Proteins -- Acid-Urea Gel Electrophoresis and Western Blotting Of Histones -- Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast -- A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae -- High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology -- Physarum Polycephalum for Studying the Function Of Histone Modifications In Vivo -- A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins. .
520			_aThis volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histones: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aGuillemette, Benoit _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGaudreau, Luc R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493966288
776	0	8	_iPrinted edition: _z9781493966295
776	0	8	_iPrinted edition: _z9781493982547
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6630-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233753 _d233753