000			03684nam a22003735i 4500
001			233759
003			ES-VaUE
005			20221220020519.0
007			cr nn 008mamaa
008			141011s2015 xxu| s |||| 0|eng d
020			_a9781493916610
024	7		_a10.1007/978-1-4939-1661-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aApoptosis and Cancer _bMethods and Protocols _cedited by Gil Mor, Ayesha B. Alvero.
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 208 páginas) _b70 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1219
505	0		_aCaspase-3 Activation is a Critical Determinant of Genotoxic Stress-induced Apoptosi -- Flow Cytometry Enumeration Of Apoptotic Cancer Cells By Apoptotic Rate -- "Multiplexed Viability, Cytotoxicity and Caspase Activity Assays" -- A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation using Live Content Imaging -- Detection of End Stage Apoptosis by ApopTag® TUNEL Technique -- Detection and Quantification of Apoptosis in Primary Cells using Taqman® Protein Assay -- Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples -- Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation -- Homogeneous, Bioluminescent Proteasome Assays -- Laser Capture Microdissection for Gene Expression Analysis -- Using the Peggy Simple Western System for Fine Needle Aspirate Analysis -- Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging -- Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles -- Measuring Cardiac Autophagic Flux in vitro and in vivo -- PET Imaging for Tyrosine Kinase Inhibitor (TKI) Bio distribution in Mice.
520			_aIn Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.
700	1		_aMor, Gil _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAlvero, Ayesha B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493916627
776	0	8	_iPrinted edition: _z9781493916603
776	0	8	_iPrinted edition: _z9781493955251
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1661-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233759 _d233759