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001			233761
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007			cr nn 008mamaa
008			130202s2013 gw | s |||| 0|eng d
020			_a9783642344107
024	7		_a10.1007/978-3-642-34410-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAnalyzing Microbes _bManual of Molecular Biology Techniques _cedited by Dilip Kumar Arora, Surajit Das, Mesapogu Sukumar.
250			_a1st edition 2013
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2013
300			_a1 recurso en línea (XI, 352 páginas) _b80 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aMicrobial DNA extraction, purification and quantitation -- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR -- Restriction enzymes and their role in microbiology -- Genetic fingerprinting techniques  for molecular characterization of microbes -- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles -- Molecular identification of microbes -- Preservation and maintenance of microbial cultures -- Microbes from extreme environment: Molecular identification procedures -- ELISA based identification and detection of microbes -- Analysis of microbial diversity and construction of metagenomic library -- Bioinformatics tools for interpretation of data used in molecular identification -- Molecular phylogenetics of microbes -- Microarray technology: Basic concept, protocols and applications -- Microarray analysis of different functional genes of microorganisms -- DNA cloning and sequencing -- Biological sequence analysis: Algorithms and statistical methods.
520			_aThis Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.
700	1		_aArora, Dilip Kumar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDas, Surajit _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSukumar, Mesapogu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783642344114
776	0	8	_iPrinted edition: _z9783642344091
776	0	8	_iPrinted edition: _z9783662509678
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-642-34410-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233761 _d233761