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001			233762
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005			20221220020520.0
007			cr nn 008mamaa
008			121025s2013 xxu| s |||| 0|eng d
020			_a9781627031257
024	7		_a10.1007/978-1-62703-125-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEpithelial Cell Culture Protocols _cedited by Scott H. Randell, M. Leslie Fulcher.
250			_a2nd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XII, 469 páginas) _b82 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v945
505	0		_aProgress Towards Drosophila Epithelial Cell Culture -- Culture of Choriod Plexus Epithelial Cells and In Vitro Model of Blood-CSF Barrier -- Methods for Culture of Human Corneal and Conjunctival Epithelia -- The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye -- Oral and Pharyngeal Epithelial Keratinocyte Culture -- Primary Culture of the Human Olfactory Neuroepithelium -- Primary Culture of Mammalian Taste Epithelium -- Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture -- Culture and Differentiation of Mouse Tracheal Epithelial Cells -- Isolation and Culture of Alveolar Epithelial Type I and Type II cells from Rat Lungs -- Classical Human Epidermal Keratinocyte Cell Culture -- Mouse Epidermal Keratinocyte Culture -- Three Dimensional Culture of Human Breast Epithelial Cells: The How and The Why -- Three Dimensional Cultures of Mouse Mammary Epithelial Cells -- Isolation, Characterisation and Re-aggregate Culture of Thymic Epithelial Cells -- Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development -- Long Term Culture and Co-Culture of Primary Rat and Human Hepatocytes -- Tissue-Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease -- Primary Mouse Small Intestinal Epithelia Cell Cultures -- Isolation, Growth and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods -- Human Female Reproductive Tract Epithelial Cell Culture -- Primary Culture and Propagation of Human Prostate Epithelial Cells -- Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Structures -- Analysis of Tissue Interactions in Ectodermal Organ Culture -- Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering -- Viral Transformation of Epithelial Cells.
520			_aFascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.
700	1		_aRandell, Scott H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFulcher, M. Leslie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627031240
776	0	8	_iPrinted edition: _z9781627031264
776	0	8	_iPrinted edition: _z9781493956791
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-125-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233762 _d233762