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001			233764
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007			cr nn 008mamaa
008			130517s2013 xxu| s |||| 0|eng d
020			_a9781627034449
024	7		_a10.1007/978-1-62703-444-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNeural Development _bMethods and Protocols _cedited by Renping Zhou, Lin Mei.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XIII, 347 páginas) _b59 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1018
505	0		_aIsolation and Maintenance of Cortical Neural Progenitor Cells In Vitro -- Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture -- Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny -- Culture of Dissociated Hippocampal Neurons -- Culturing Mouse Cerebellar Granule Neurons -- Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain -- Culturing Astoryctes from Postnatal Rats -- Culturing Oligodendrocyte Lineage Cells from Neonatal Rats -- Isolation and Culture of Schwann Cells -- DNA Transfection: Calcium Phosphate Method -- The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices -- Lentiviral Vector Production, Titration, and Transduction of Primary Neurons -- In-Ovo Electroporation in Embryonic Chick Spinal Cords -- Gene Transfer in Developing Chick Embryos: In Ovo Electroporation -- In Utero Electroporation in Mice -- General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections -- Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections -- β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations -- Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei -- In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System -- Growth Cone Collapse Assay -- The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro -- Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture -- Semi-Automated Analysis of Dendrite Morphology in Cell Culture -- Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines -- Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms- Routine Histology Techniques for the Developing and Adult Central Nervous System -- Cryosectioning -- The Golgi-Cox Method -- Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine -- Retrograde Tracing Technique for Neonatal Animals.
520			_aUnderstanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.
700	1		_aZhou, Renping _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMei, Lin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034456
776	0	8	_iPrinted edition: _z9781627034432
776	0	8	_iPrinted edition: _z9781493960187
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-444-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233764 _d233764