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001			233772
003			ES-VaUE
005			20221220020520.0
007			cr nn 008mamaa
008			160616s2016 gw | s |||| 0|eng d
020			_a9783662504321
024	7		_a10.1007/978-3-662-50432-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bSynthetic and Systems Biology - Tools _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2016
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2016
300			_a1 recurso en línea (X, 199 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Protocol for the standardisation of transcriptional measurements -- Uracil-excision for assembly of complex pathways -- Quantitative physiology approaches to understand and optimize reducing power availability in environmental bacteria -- Design of orthogonal pairs for protein translation: selection systems for genetically encoding noncanonical amino acids in E. coli -- Phenome-ing microbes -- Systems biology tools for methylotrophs -- Protocols for probing genome architecture of regulatory networks in hydrocarbon and lipid microorganisms -- A practical protocol for genome-scale metabolic reconstruction -- Computer-guided metabolic engineering -- Improving biocontainment with synthetic biology: beyond physical containment.
520			_aThis Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise "reducing power availability", and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662504307
776	0	8	_iPrinted edition: _z9783662504314
776	0	8	_iPrinted edition: _z9783662570586
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-50432-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233772 _d233772