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001			233773
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007			cr nn 008mamaa
008			130823s2013 xxu| s |||| 0|eng d
020			_a9781627036405
024	7		_a10.1007/978-1-62703-640-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNeuronal Cell Culture _bMethods and Protocols _cedited by Shohreh Amini, Martyn K. White.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 165 páginas) _b38 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1078
505	0		_aGeneral Overview of Neuronal Cell Culture -- Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology -- Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12 -- Murine Teratocarcinoma-Derived Neuronal Cultures -- Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons -- Mouse Enteric Neuronal Cell Culture -- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications -- Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA) -- Slice Culture Modeling of Central Nervous System (CNS) Viral Infection -- Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping -- Transfection of Neuronal Cultures -- Lentiviral Transduction of Neuronal Cells -- Compartmentalized Neuronal Cultures -- Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates.
520			_aIn Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.  Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
700	1		_aAmini, Shohreh _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWhite, Martyn K _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627036412
776	0	8	_iPrinted edition: _z9781627036399
776	0	8	_iPrinted edition: _z9781493960644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-640-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233773 _d233773