000			04622nam a22003855i 4500
001			233780
003			ES-VaUE
005			20221220020521.0
007			cr nn 008mamaa
008			161219s2017 gw | s |||| 0|eng d
020			_a9783662527788
024	7		_a10.1007/978-3-662-52778-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bMicrobial Quantitation, Community Profiling and Array Approaches _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 252 páginas) _b30 ilustraciones, 12 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction to Microbial Quantitation, Community Profiling, and Array Approaches -- A cell extraction method for oily sediments -- Introduction to microplate MPN-enumeration of hydrocarbon degraders -- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs -- Microbial biomass and community analysis by PLFA analysis -- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities -- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities -- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis -- Microbial community profiling: SSCP and T-RFLP techniques -- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) - preparation, data handling and ecological analysis -- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment -- Studying protistan communities in hydrocarbon-contaminated environments.
520			_aThis Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662527764
776	0	8	_iPrinted edition: _z9783662527771
776	0	8	_iPrinted edition: _z9783662570814
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-52778-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233780 _d233780