000			04903nam a22003615i 4500
001			233782
003			ES-VaUE
005			20221220020521.0
007			cr nn 008mamaa
008			110414s2009 xxu| s |||| 0|eng d
020			_a9781597451901
024	7		_a10.1007/978-1-59745-190-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChromatin Protocols _cedited by Srikumar P. Chellappan.
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XIV, 416 páginas) _b51 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v523
505	0		_aPreparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach -- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins -- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules -- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of DNA Topology in Yeast Chromatin -- Preparation and Analysis of Uniquely Positioned Mononucleosomes -- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy -- Methods to Study Transcription-Coupled Repair in Chromatin -- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs) -- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- In Vitro Replication Assay with Mammalian Cell Extracts -- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction -- Biochemical Analysis of Arginine Methylation in Transcription -- In Vitro Histone Demethylase Assays -- Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation -- Histone Deacetylase Activity Assay -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response.
520			_aSignificant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
700	1		_aChellappan, Srikumar P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607612520
776	0	8	_iPrinted edition: _z9781588298737
776	0	8	_iPrinted edition: _z9781493956265
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-190-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c233782 _d233782