000			04104nam a22003615i 4500
001			233786
003			ES-VaUE
005			20221220020521.0
007			cr nn 008mamaa
008			170327s2017 xxu| s |||| 0|eng d
020			_a9781493963409
024	7		_a10.1007/978-1-4939-6340-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMeiosis _cedited by David T. Stuart.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 337 páginas) _b59 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1471
505	0		_aGenetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae -- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe -- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast -- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis -- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation -- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora -- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae -- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast -- In Vivo Imaging of Budding Yeast Meiosis -- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I -- Flow Cytometry for the Isolation and Characterization of Rodent Meiocytes -- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH -- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy -- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes -- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes -- Drosophila Male Meiosis -- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis -- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model -- A Computational Approach to Study Gene Expression Networks.
520			_aThis updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
700	1		_aStuart, David T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493963386
776	0	8	_iPrinted edition: _z9781493963393
776	0	8	_iPrinted edition: _z9781493981694
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6340-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233786 _d233786