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020 _a9781493914388
024 7 _a10.1007/978-1-4939-1438-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aParasite Genomics Protocols
_cedited by Christopher Peacock.
250 _a2nd edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XII, 367 páginas)
_b49 ilustraciones, 34 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1201
505 0 _aThe Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites -- From Sequence Mapping to Genome Assemblies -- Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths -- A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome -- Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes -- Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes -- Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in Leishmania -- Gene Suppression in Schistosomes using RNAi -- Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends -- Techniques to Study Epigenetic Control and the Epigenome in Parasites -- The Genome-wide Identification of Promoter Regions in Toxoplasma gondii -- RNA-seq Approaches for Determining mRNA Abundance in Leishmania -- Protein Microarrays for Parasite Antigen Discovery -- A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa -- Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions -- Proteomic Analysis of Post Translational Modifications using iTRAQ in Leishmania -- Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug Treatment -- Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites -- Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics -- Screening Leishmania donovani Complex-specific Genes Required for Visceral Disease.
520 _a Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era.  With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale 'omics' methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
700 1 _aPeacock, Christopher
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493914395
776 0 8 _iPrinted edition:
_z9781493914371
776 0 8 _iPrinted edition:
_z9781493942206
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1438-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c233792
_d233792