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020 _a9781627032513
024 7 _a10.1007/978-1-62703-251-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aDopamine
_bMethods and Protocols
_cedited by Nadine Kabbani.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 312 páginas)
_b78 ilustraciones, 22 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v964
505 0 _aDetection of Cell Surface Dopamine Receptors -- Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells -- Methods of Dopamine Research in Retina Cells -- Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis -- Modeling Spatial Aspects of Intracellular Dopamine Signaling -- A Biophysical Approach for the Study of Dopamine Receptor Oligomerization -- Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology -- BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling -- Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging -- Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6 -- A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms -- Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish -- Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila -- Regulation of Dopamine Transporter Expression by Neuronal Activity -- Monitoring Somatodendritic and Axonal Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices -- Real-Time Chemical Measurements of Dopamine Release in the Brain -- The MPTP/Probenecid Model of Progressive Parkinson's Disease.
520 _aDopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.  In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson's disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
700 1 _aKabbani, Nadine
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627032520
776 0 8 _iPrinted edition:
_z9781627032506
776 0 8 _iPrinted edition:
_z9781493963331
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-251-3
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c233794
_d233794