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001			233797
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007			cr nn 008mamaa
008			151031s2016 xxu| s |||| 0|eng d
020			_a9781493929665
024	7		_a10.1007/978-1-4939-2966-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTGF-β Signaling _bMethods and Protocols _cedited by Xin-Hua Feng, Pinglong Xu, Xia Lin.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 394 páginas) _b64 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1344
505	0		_aRegulation of TGF-b Receptors -- Determining TGF-β Receptor Levels in the Cell Membrane -- Post-translational Modifications of TGF-b Receptors -- Production, Isolation，and Structural Analysis of Ligands and Receptors of the TGF-b Superfamily -- Phosphorylation of Smads by Intracellular Kinases -- Analysis of Smad Phosphatase Activity in vitro -- Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b Signaling -- TGF-β Signaling in Stem Cell Regulation -- Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β -- In vitro Th differentiation protocol -- Interrogating TGF-b Function and Regulation in Endothelial Cells -- Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily Functions -- Imaging TGFb Signaling in Mouse Models of Cancer Metastasis -- Generation and Characterization of Smad7 Conditional Knockout Mice -- Monitoring Smad Activity in vivo Using the Xenopus Model System -- Animal Cap Assay for TGF-b Signaling -- Detection of Smad Signaling in Zebrafish Embryos -- Role of TGF-β signaling in Coupling Bone Remodeling -- Studying the Functions of TGF-β Signaling in the Ovary -- Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated by TGF-b Signaling -- TGF-β Regulated microRNAs and Their Function in Cancer Biology -- Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras Activation -- The Role of Ubiquitination to Determine Non-Smad Signaling Responses -- Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction Pathway -- Measuring TGF-β Ligand Dynamics in Culture Medium.
520			_aThis volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aFeng, Xin-Hua _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aXu, Pinglong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLin, Xia _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929658
776	0	8	_iPrinted edition: _z9781493929672
776	0	8	_iPrinted edition: _z9781493949939
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2966-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233797 _d233797