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020 _a9781493926978
024 7 _a10.1007/978-1-4939-2697-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Gravitropism
_bMethods and Protocols
_cedited by Elison B. Blancaflor.
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XIII, 323 páginas)
_b93 ilustraciones, 69 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1309
505 0 _aImmunohistochemistry Relative to Gravity: A Simple Method to Retain Information About Gravity for Immunolocalization and Histochemistry -- A Flat Embedding Method to Orient Thin Biological Samples for Sectioning -- Quantification of Root Gravitropic Response Using a Constant Stimulus Feedback System -- The Analysis of Gravitropic Setpoint Angle Control in Arabidopsis -- Imaging of Dynamic Ion Signaling During Root Gravitropism -- Live Cell Imaging of Cytoskeletal and Organelle Dynamics in Gravity-Sensing Cells in Plant Gravitropism -- Auxin Carrier and Signaling Dynamics During Gravitropic Root Growth -- Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements during Root Development and Gravitropism -- Methods for RNA Profiling of Gravi-Responding Plant Tissues -- Proteomic Approaches and their Application to Plant Gravitropism -- Assays for Root Hydrotropism and Response to Water Stress -- Evaluating Mechano-Transduction and Touch Responses in Plant Roots -- Expressing and Characterizing Mechanosensitive Channels in Xenopus Oocytes -- Flowering Shoots of Ornamental Crops as a Model to Study Cellular and Molecular Aspects of Plant Gravitropism -- Studying Molecular Changes During Gravity Perception and Response in a Single Cell -- Live Cell and Immuno-Labeling Techniques to Study Gravitational Effects on Single Plant Cells -- Use of High Gradient Magnetic Fields to Evaluate Gravity Perception and Response Mechanisms in Plants and Algae -- Use of Microgravity Simulators for Plant Biological Studies -- Conducting Plant Experiments in Space -- Spaceflight Exploration in Plant Gravitational Biology -- Hypergravity Experiments to Evaluate Gravity Resistance Mechanisms in Plants.
520 _aThis volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.
700 1 _aBlancaflor, Elison B
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493926961
776 0 8 _iPrinted edition:
_z9781493926985
776 0 8 _iPrinted edition:
_z9781493955817
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2697-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c233798
_d233798