000			04004nam a22003615i 4500
001			233801
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005			20221220020522.0
007			cr nn 008mamaa
008			160517s2016 xxu| s |||| 0|eng d
020			_a9781493936700
024	7		_a10.1007/978-1-4939-3670-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aWest Nile Virus _bMethods and Protocols _cedited by Tonya M. Colpitts.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 295 páginas) _b32 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1435
505	0		_aA Brief Review of West Nile Virus Biology. Overview of West Nile Virus Transmission and Epidemiology -- Propagation and Titration of West Nile Virus on Vero Cells -- Generating West Nile Virus from an Infectious Clone -- Functional Analysis of West Nile Virus Proteins in Human Cells -- Lessons from the Murine Models of West Nile Virus Infection -- Exploration of West Nile Virus Infection in Mouse Models -- Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model -- In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis -- Methodology for Identifying Host Factors Involved in West Nile Virus Infection -- ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses -- Safe Handling of West Nile Virus in the Insectary -- Techniques for Experimental Infection of Mosquitoes with West Nile Virus -- Field Surveillance Methods for West Nile Virus -- Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Non-Human Mammals -- Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian Hosts -- Statistical Tools for the Interpretation of Enzootic West Nile Virus Transmission Dynamics -- Role of Immune Aging in Susceptibility to West Nile Virus -- An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection.
520			_aThis volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, and in vitro and in vivo blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful Methods in Molecular Biology series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, West Nile Virus will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.
700	1		_aColpitts, Tonya M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936687
776	0	8	_iPrinted edition: _z9781493936694
776	0	8	_iPrinted edition: _z9781493981120
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3670-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233801 _d233801